Objective In this study, 2, 4-dinitrochlorobenzene ( DNCB ) was used to induce the establishment of an atopic dermatitis (AD) model in BALB/ c homozygous mice to simulate the skin inflammatory complications in patients with clinical malignancies. Methods BALB/ c mice were divided into different groups:negative control group ( NC group), model group ( MODEL group), atopic dermatitis group ( AD group), and dexamethasone group (DEX group). After the mice in MODEL group and DEX group were inoculated with S-180 tumor cells in the axilla, MODEL group, AD group and DEX group were stimulated with DNCB on the dorsal skin and the ear to establish an animal model of atopic dermatitis in tumor-bearing mice. Changes in body weight were observed and recorded, the dorsal skin condition of mice was assessed after the last administration of the drug, the spleen was taken to calculate the spleen coefficient, the difference in the mass of mouse ear slices was determined to calculate the degree of auricular swelling and the rate of inhibition of swelling, and histopathological tests were performed on the dorsal skin tissues to detect the levels of IgE, TNF-α, IL-4, and IL-17 in the serum using an ELISA assay. Results Compared with the NC group, the skin of mice in the MODEL and AD groups showed erythematous, papular, scaly and mossy changes, accompanied by weight loss, and a significant increase in splenic coefficient and auricular swelling. Pathologic findings showed an incomplete skin structure, a significant increase in skin thickness, a large infiltration of inflammatory cells, and an increase in the number of mast cells. Serum levels of IgE, TNF-α, IL-4 and IL-17 were increased. Compared with the MODEL group, the DEX group showed an improvement in all the assays. Conclusions DNCB excitation can successfully establish an animal model of AD in hormonal mice, which is drugcontrollable, which provides a useful scientific tool for conducting scientific research related to malignant tumors and skin inflammation.