乳腺癌荷瘤裸鼠模型证候属性和姜制江香薷多糖的影响
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1. 江西中医药大学实验动物科技中心,南昌 330004;2. 江西中医药大学临床医学院,南昌 330004;3. 南昌市实验动物病理重点实验室,南昌 330004

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Impact of ginger-processed Jiangxiangru polysaccharides on establishment of a breast cancer tumor-bearing nude mice model and its pathological characteristics
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1. Science & Technology Center for Experimental Animal of Jiangxi University of Chinese Medicine, Nanchang 330004, China; 2. Clinical Medical College of Jiangxi University of Chinese Medicine, Nanchang 330004, China; 3. Key Laboratory of Pathological Research on Experimental Animals of Nanchang, Nanchang 330004, China

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    摘要:

    目的 观察乳腺癌荷瘤裸鼠病症结合特点及姜制江香薷多糖对其中医证候的改善作用。 方法 取38只实验裸鼠进行造模然后分成6组,设定为正常组、模型组、阳性组、姜制江香薷多糖低、中、高剂量组。正常组不接种肿瘤,正常组和模型组给予生理盐水灌胃;阳性组给予塞来昔布溶液灌胃;姜制江香薷多糖低、中、高剂量组给予同剂量不同浓度姜制江香薷多糖溶液灌胃。连续给药4周,记录裸鼠体质量和肿瘤大小变化。实验结束后观察裸鼠的病症特点,通过采集各组裸鼠舌、尾、爪照片,分析记录裸鼠舌、尾、爪的RGB值依据计量化辩证的方法计算裸鼠的血虚程度、阴虚程度、肿瘤痰瘀邪盛程度,剥离肿瘤,称重,计算肿瘤体积和抑瘤率,探究姜制江香薷多糖对乳腺癌荷瘤裸鼠中医证候的改善作用。 结果 乳腺癌荷瘤裸鼠存在血虚、阴虚和痰瘀邪盛表现,姜制江香薷多糖给药组可以明显减少肿瘤的大小,姜制江香薷多糖能够抑制肿瘤生长,对乳腺癌荷瘤裸鼠血虚程度、阴虚程度、肿瘤痰瘀邪盛体质有改善效果,且姜制江香薷多糖高剂量组最佳。 结论 姜制江香薷多糖对乳腺癌荷瘤裸鼠的血虚、阴虚、肿瘤痰瘀邪盛体质有改善作用,且高剂量组效果最佳。

    Abstract:

    Objective To observe the characteristic symptoms in breast cancer-bearing mice and the beneficial effect of ginger-processed Jiangxiangru polysaccharides on traditional Chinese medical symptoms. Methods Thirty-eight mice were used for modeling and divided into normal, model, positive, and low-, medium-, and highdose ginger-processed Jiangxiangru polysaccharide groups. Mice in the normal group were not inoculated with tumors and mice in the normal and model groups received physiological saline intragastrically. Mice in the positive group received celecoxib solution intragastrically, and mice in the low-, medium-, and high-dose groups received the same dose but different concentrations of ginger-processed Jiangxiangru polysaccharide solution intragastrically. Changes in body weight and tumor size were recorded after 4 weeks of continuous administration. Symptoms were observed at the end of the experiment. RGB values in photographs of the tongues, tails, and claws from mice in each group were analyzed and recorded. The degrees of blood deficiency, yin deficiency, and tumor phlegm stasis were calculated based on the method of quantitative dialectical diagnosis. The tumors were isolated and weighed, and the tumor volume and inhibition rate were calculated to determine the beneficial effect of ginger-processed Jiangxiangru polysaccharides on traditional Chinese medical symptoms. Results Mice in the breast cancer model group showed signs of blood deficiency, yin deficiency, and phlegm stasis. Tumor size was significantly reduced in mice in the ginger-processed Jiangxiangru polysaccharide groups. Ginger-processed Jiangxiangru polysaccharides inhibited tumor growth and improved blood deficiency, yin deficiency, and tumor phlegm stasis in breast cancer-bearing mice, with the best result in the high-dose group. Conclusions Ginger-processed Jiangxiangru polysaccharides can improve the symptoms of blood deficiency, yin deficiency, and tumor phlegm stasis in breast cancer-bearing mice, especially at high doses.

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黄帅,马秋婷,洪滔,钟祥铭,张文凯,黄云,刘志勇.乳腺癌荷瘤裸鼠模型证候属性和姜制江香薷多糖的影响[J].中国实验动物学报,2025,33(5):656~664.

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  • 收稿日期:2024-08-24
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  • 在线发布日期: 2025-07-08
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