Myofascial pain syndrome (MPS) is a musculoskeletal disorder involving skeletal muscle and surrounding fascia. It is characterized by localized pain, the presence of myofascial trigger points(MTrPs) within muscle tissue, and abnormal spontaneous electrical activity. To investigate the pathological mechanisms of MPS and to develop novel therapeutic approaches, the establishment of reliable and reproducible animal models is essential.Based on the clinical and pathological features of MPS, a range of animal modeling method have been developed,including electrical stimulation, pharmacological induction, and mechanical injury combined with eccentric exercise.This review summarizes current mainstream animal models of MPS, discusses their establishment method and applicable scenarios, and analyzes the strengths and limitations of existing model evaluation systems. In addition, a multidimensional and Objective evaluation strategy is proposed to support model standardization and to facilitate the development of animal models that more closely reflect the pathogenesis of MPS, thereby providing a reference for future in-depth research.