摘要 目的 建立小鼠原发性痛经模型。方法 不同剂量补佳乐给近交系BALB/c小鼠连续灌胃，末次给药后腹腔注射缩宫素，诱发扭体反应，记录扭体潜伏期和扭体次数，筛选最佳条件。结果 补佳乐最佳剂量为0.5 mg?kg-1；催产素最佳剂量为2U/只；补佳乐灌胃后1个小时为观察扭体反应的最佳时间，用药周期第3天时扭体次数开始增多；从第4天开始维持在高水平。结论 补佳乐联合缩宫素可以成功建立小鼠原发性痛经模型。
Abstract: Aim To set up primary dysmenorrheal model in mice with progynova and oxytocin; Methods Administrated different doses of progynova in mice continuously and after last dosage peritoneal injected oxytocin to induce writhing response, recorded the writhing latency period and frequency. Results: The optimum dose of progynova was 0.5 mg?kg-1and oxytocin 2U.1hour after administrated progynova was the optimal time to observe writhing response. From the third day writhing frequency begin to increase and maintain high level from the fourth day. Conclusion: progynova combined with oxytocin could build primary dysmenorrheal model.
陈景伟,仝瑞晓,孙晓换,范丽洁,杜惠兰.补佳乐联合缩宫素建立小鼠原发性痛经模型[J].中国实验动物学报,2013,21(5).
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