目的 通过调整窝仔数的方法建立幼儿单纯性肥胖模型，并与高脂饲料制备模型进行比较。方法 48只雌性KM小鼠产仔鼠后，一半仔鼠数目为14~16只，一半仔鼠调整为6只（雌雄各3只）。仔鼠断乳时或第9周时仔鼠分别饲喂普通饲料或高脂饲料。仔鼠在15周后处死，称重，测量体长、腰围，生殖器重、脂肪重（肾周和生殖器周脂肪），计算体脂比。结果 ①BD2组常规饲料饲喂至15周结束后，无论雌雄仔鼠，其体重与BD1组比较均显著性差异（P均<0.05），且BD2组体重超过BD1组体重雌性为26.3%，雄性为20.0%。同一处理方式，雌性仔鼠体脂比均高于雄性。②不同时间饲喂高脂饲料，无论调整窝仔数与否，高脂饲料各组仔鼠，无论雌雄，其各组仔鼠体重均比BD1组高（P均<0.05）；HFD4组雌性和雄性仔鼠体重与BD2组相比存在均显著差异（P均<0.05）。结论 通过调整窝仔数的方法可以成功制备儿童单纯性肥胖模型。在儿童早期肥胖的情况下，成年后过渡高脂饮食会导致机体储存更多的脂肪。
[Abstract]Objective To make the childhood simple obesity animal model by adjusting the litter size of offspring and compare with the obesity model by high fat diet.Methods After 48 female KM mice farrowing, the litter size of half of them remain 14-16, the other adjust to 6 (3 male and 3 female).Pups were fed with basic diet and high fat at 4 or 9 week old and continued to the fifteen week.Weight, body length, waist circumference, weight of genital organ, fat weight including perirenal fat and genital organ fat were weighed and the ratio of body fat were calculated.Results ①There was significantly difference in the female or male body weight between BD1 and BD2 group feeding the basic diet for 15 weeks (P<0.05), and the body weight of BD2 were over 26.3%(female), 20%(male). The ratio of body fat of female in each group were higher than male’s under the same treatment methods.② No matter when we changed the diet or whether we adjusted the litter size or not, the body weight of newborn rats feeding HFD, both male and female, were higher than BD1 group (all P<0.05).Female and male offspring rats body weight in HFD4 group had significant higher than BD2 group (all P<0.05).Conclusion Childhood simple obesity animal model could be successfully prepared by adjusting the litter size.They began to intake high fat diet at adult stage,who got childhood simple obesity,would make the body store more fat.
陈丽.儿童单纯性肥胖模型的制作[J].中国实验动物学报,2013,21(5).
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