【Abstract】 Objective To establish an effective way for intratracheal siRNA delivery to the mouse airway. Methods Female BALB/c mice were used to establish asthma model by egg albumin hypersensitivity. Asthma mice were then randomly divided into control group and siRNA group. mice in siRNA group were intratracheally adminstrated with NF-κB p50 siRNA (20uM，100ul/per mouse) by aerosolizer while mice in control group were administrated negative control siRNA.after administration, lung tissue were collected for analysis of NF-κB expression by Real time PCR. Results The expression of NF-κB gene was reduced by 1.743 fold after intratracheal aerosol administration of NF-κB siRNA compared to control group（P=0.0035）. Conclusion Intratracheal aerosol administration of siRNA was an effective method to inhibit gene expression in mice airway.