Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders, of which diarrhea-predominant IBS (IBS-D) accounts for the largest proportion. The pathogenesis of IBS-D is complicated and diverse, and there is currently a lack of clinically effective drugs. The establishment of animal models is an essential tool for further studies of the disease mechanisms, evaluation of clinical efficacy, and drug development, and the preparation and evaluation standards of models are important factors affecting the quality of the research. Based on the currently accepted pathogenesis of IBS-D and the previous modeling experience of our research group, this review systematically summarizes the evaluation methods used in animal models of IBS-D in terms of diarrhea observation, visceral sensitivity tests, and intestinal motility tests, to provide a reference for future studies.