【Abstract】Objective To study the characteristics and application status of animal models of ovarian cancer and provide guidance for standardized preparation of ovarian cancer models. Methods Using “ovarian cancer” and “animal model” as the main topics in CNKI, Wanfang, VIP, and PubMed databases, the databases were searched for animal experimentation literature on ovarian cancer from establishment of the database to March 1, 2023. A total of 1428 relevant articles were collected. From the experimental animal species, age, experimental modeling methods, detection indicators, and other aspects of the summary, we established a database for systematic analysis. Results A total of 178 studies were obtained after screening. Female BALB/c mice were selected to establish the animal model of ovarian cancer. Most choices were 4 ~ 6 and 6 ~ 8 weeks of age. The most common modeling method was ectopic transplantation, which was mostly subcutaneous injection in the axilla. In terms of modeling methods of ovarian cancer in animals, transplantable models were the most used, and ectopic transplantation was more often employed than orthotopic transplantation. The ovarian cancer cell lines used were mostly human. Most detection indexes were the appearance of tumor tissue, tumor pathology, and immunohistochemistry. Conclusion Animal models of ovarian cancer are widely used in ovarian cancer research, but standard preparation and evaluations are lacking. We analyzed the application status of animal models of ovarian cancer in detail through literature collation and data classification analysis to provide a reference to establish standardized animal models of ovarian cancer. BALB/c mice are the most selected, and human ovarian cancer cell line SKOV3 at 1 × 107 cells/ml is commonly injected into the subcutaneous axillary area for 10 days. A large number of models can be obtained in a short time with a high tumor formation rate and small individual differences, which provide a reference to study ovarian cancer.