芩蓟凉血合剂对痔病相近动物模型的影响
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上海中医药大学附属市中医医院肛肠科

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:上海市医院中药制剂产业转化协同创新中心,上海中医药大学产业发展中心医养结合科创项目(YYKC-2021-01-140),上海市中医医院未来计划(WLJH2021ZY-MZY024,WLJH2021ZY-GZS003,WLJH2021ZY-MZY024),上海市卫生健康委员会(ZY(2021-2023)-0204-01),上海市卫生健康委员会中医药科研项目计划(2022QN011)。


Effect of Qinji Liangxue mixture on similar animal models of hemorrhoids
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Department of Anorectal Surgery,Shanghai Municipal Hospital of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai,200071 China

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    摘要:

    目的 探讨芩蓟凉血合剂(QLM)对痔病相近动物模型的影响。方法 分别使用巴豆油制剂(COP)、冰醋酸制备大鼠急性痔病动物模型,将80只SD雌性大鼠,随机分为对照组,模型组,QLM低剂量组(QLM-L),QLM高剂量组(QLM-H),地奥司明组(DOSM),每组8只,除对照组,其余组均使用COP或冰醋酸处理,随后使用不同药物治疗7天。使用HE染色观察COP及冰醋酸诱导的肛门直肠组织形态学,使用ELISA检测COP诱导的大鼠血清IL-8、TNF-α水平,记录冰醋酸诱导的大鼠肛周溃疡面积。将24只C57小鼠随机分为对照组,QLM-L组,QLM-H组,肾上腺色腙片组(CT),每组6只,连续治疗7天,使用毛细管法及断尾法评价QLM的止血疗效。结果 QLM能够显著改善痔病相近动物模型的病理损伤,减轻COP诱导的病变程度评分(P<0.05),降低血清TNF-α、IL-8水平(P<0.05),减少冰醋酸诱导的肛周溃疡面积(P<0.05),缩短凝血及出血时间(P<0.05)。结论 QLM具有良好的抗痔活性,其作用可能是通过抗炎、止血、减少组织损伤实现的。

    Abstract:

    Objective To explore the effect of Qinji Liangxue mixture (QLM) on similar animal models of hemorrhoid. Methods Animal models of acute hemorrhoid in rats were made by using croton oil preparation (COP) and glacial acetic acid respectively. Eighty SD female rats were randomly divided into control group, model group, low-dose QLM group (QLM-L), high-dose QLM group (QLM-H) and diosmin group (DOSM), with 8 rats in each group. Except for the control group, all the other groups were treated with COP or glacial acetic acid, and then treated with different drugs for 7 days. HE staining was used to observe the anorectal histomorphology induced by COP and glacial acetic acid, and ELISA was used to detect the levels of IL-8 and TNF-α in serum of rats induced by COP, and the area of perianal ulcer induced by glacial acetic acid was recorded. Twenty-four C57 mice were randomly divided into control group, QLM-L group, QLM-H group and adrenal chromazone tablet group (CT), with 6 mice in each group for 7 days. The hemostatic effect of QLM was evaluated by capillary method and tail cutting method. Results QLM can significantly improve the pathological injury of animal models with similar hemorrhoid diseases, reduce the score of pathological changes induced by COP (P < 0.05), reduce the levels of serum IL-8 and TNF-α(P < 0.05),, reduce the area of perianal ulcer induced by glacial acetic acid (P < 0.05),, and shorten the time of coagulation and bleeding(P < 0.05). Conclusions QLM has good anti-hemorrhoid activity, which may be achieved by anti-inflammation, hemostasis and reducing tissue damage.

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  • 收稿日期:2023-08-28
  • 最后修改日期:2024-01-02
  • 录用日期:2024-01-12
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