Objective To investigate the protective effect and mechanism of BBD on Doxorubicin (Dox) induced myocardial injury in zebrafish. Method A zebrafish model of myocardial injury induced by chemotherapy drug Dox was established. The effects of BBD at different concentrations on pericardial edema ratio and heart rate were observed under a stereo microscope. Transgenic zebrafish Tg(mpx:EGFP) was used to observe the inhibitory effect of BBD on neutrophil infiltration in the heart in situ., and observe the effect of BBD on SOD, CAT and MDA. Real-time qPCR was used to detect the expression of ferroptosis related factors gpx4a, ptgs2, alox5a, acsl4. The accumulation of ferrous ion in zebrafish heart was detected using a ferrous ion fluorescent probe. Results BBD significantly improved Dox induced pericardial edema, heart rate and neutrophil infiltration in zebrafish (P<0.05), significantly increased the reduction of SOD and CAT activity (P<0.05), and decreased the concentration of MDA (P<0.05). Compared with the Dox group, the Dox group showed significantly increased expression of gpx4a (P<0.05) and decreased expression of ptgs2, alox5a, and acsl4 (P<0.05), and significantly inhibited Dox induced ferroptosis in zebrafish hearts. Conclusion BBD can attenuate Dox induced myocardial injury in zebrafish by inhibiting the occurrence of lipid peroxidation and regulating ferroptosis.