【】 Objective To investigate the effect of ethanolic extracts of chicory on the fecal bile acid profile of high-fat diet-induced obese mice using targeted metabolomics technology based on UPLC-Orbitrap-MS/MS. Methods Twenty-four 6-week-old C57 BL/6 male mice were selected and randomly divided into normal group, model group, drug administration group and metformin group. The normal group was given a regular diet, and the remaining three groups were fed daily with high-fat chow for 10 weeks to create an obesity model. After successful modeling, the dosed group was gavaged with 200 mg/kg ethanolic extract of Cichorium glandulosum Boiss. et Huet. daily and the metformin group was gavaged with 100 mg/kg metformin daily for 10 weeks.After 10 weeks, liver tissues were collected from the mice for the detection of hepatic total triglycerides (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C); The bile acid metabolic profiles of mice in each group were further examined by ultra-high liquid chromatography-mass spectrometry (UPLC-MS/MS) in collected feces; Results Compared with the normal group, body weight (P<0.0001) and hepatic TG (P<0.05) of the model group mice were significantly elevated, while TC showed a trend of increasing (P>0.05), LDL-C showed a trend of decreasing (P>0.05), and HDL-C was significantly decreased (P<0.001),showing weight gain and abnormal lipid metabolism. The alcoholic extract of Cichorium glandulosum Boiss. et Huet.significantly reduced body weight (P<0.0001) and significantly decreased liver TG (P<0.0001) and LDLC (P<0.05) in mice. The results of methodological validation indicated that the UPLC-Orbitrap-MS/MS method established in this study could accurately quantify 52 bile acids in feces. Analysis of the concentration of each type of bile acids revealed that the alcoholic extract of Cichorium glandulosum Boiss. et Huet. significantly increased the secondary bile acid/primary bile acid ratio (P<0.05). The results of multivariate statistical analysis showed significant changes in bile acid metabolism pattern between normal, model, administered and metformin groups. Screening for differential bile acids in the administered group relative to the model group with VIP>1 and P<0.05 showed that the alcoholic extract of Cichorium glandulosum Boiss. et Huet.significantly elevated Lithocholic Acid in fecal bile acids (P<0.001, VIP=2.187), 23-Nordeoxycholic Acid (P<0.05, VIP=1.391), and significantly decreased Deoxycholic Acid in fecal bile acids (P<0.05, VIP=1.78). VIP=1.78), 3-Epideoxycholic acid (P<0.01, VIP= 1.881), Isolithocholic Acid(P<0.05, VIP=1.705),Cholic Acid-3-Sulfate (P<0.05, VIP=1.581), ω-Muricholic Acid(P<0.05, VIP=1.533), and Glycochenodeoxycholic Acid-3-O-β-glucuronide(P< 0.05, VIP=1.516), and by searching the KEGG database, it was found that differential bile acids were mostly involved in the secondary bile acid biosynthesis pathway. Correlation analysis showed that four differential bile acids, deoxycholic acid (rs=0.6445, P<0.001), Isolithocholic Acid (rs=0.5879, P<0.01), 3β-deoxycholic acid (rs=0.6649, P<0.001) and ω-Muricholic Acid (rs=0.5387, P<0.01), in feces were strongly and positively correlated with body weight. Conclusion Cichorium glandulosum Boiss. et Huet.alcohol extract exerts weight loss and ameliorates lipid abnormalities by regulating fecal bile acid metabolism.This may be due to the fact that Cichorium glandulosum Boiss. et Huet. alcohol extract regulates secondary bile acid biosynthesis and exerts a fat-reducing effect.