Objective Establishment and evaluation of aldosterone-induced multiple-organ damage mouse model. Methods Twenty mice were randomly divided into four groups, with five mice per group: blank control Group A given 0 μg/(kg·d), low-dose aldosterone model Group B given 150 μg/(kg·d), medium-dose aldosterone model Group C given 300 μg/(kg·d), and high-dose aldosterone model Group D given 450 μg/(kg·d). An osmotic micro-pump containing aldosterone was surgically implanted under the skin that infused the mice with aldosterone for four weeks to establish an aldosterone damage model. The body weight and blood pressure of the mice were recorded weekly. After four weeks of modeling, blood pressure and histology in various organs were observed and analyzed. Results (1) After four weeks of infusion with aldosterone, the serum aldosterone levels of mice in groups C and D significantly increased, whereas there was no significant increase in Group B. (2) After the mice were implanted with osmotic pumps, the systolic blood pressure in each model group increased significantly in the second and third weeks, but in the fourth week, the blood pressure in the model groups decreased. (3) Renal and cardiac injuries, interstitial edema, collagen deposition, and fibrosis occurred to varying degrees in each model group. A small amount of collagen deposition occurred in the liver of Group B, and liver cell injury, collagen deposition, and fibrosis occurred to varying degrees in groups C and D. Conclusions Aldosterone can induce multi-organ damage in mice. Under this modeling method, organ damage mainly manifests as edema, collagen deposition, and fibrosis lesions.