Objective To explore the way to establish and evaluate the asthma rat model with phlegm and blood stasis syndrome. Methods 60 SD male rats were randomly divided into 5 groups:normal group,asthma model group,combination of disease and syndrome group(combination group),dexamethasone group,Kechuan Liuwei Mixture group),with 12 rats in each group. Asthma models were established by inducing ovalbumin (OVA) . Syndrome model of phlegm and blood stasis was established using a high-fat diet combined with ice water bath method. Evaluate asthma model through animal behavior observation,pathological section observation,inflammation index detection,and airway reactivity measurement; Evaluate phlegm and blood stasis syndrome model by measuring rat body mass,observing symptoms,detecting blood glucose,blood lipids,coagulation function,and hemorheological indexes,as well as syndrome determination by Kechuan Liuwei Mixture. Results ① After OVA inducing,the rats in the asthma model group and combination group showed symptoms such as shortness of breath,open mouth breathing,abdominal movement,restlessness,and irritability; HE staining showed disordered arrangement of the bronchial mucosa in lung tissue,local detachment,thickening of the basement membrane and the bronchial tube wall,narrowing of the lumen,extensive infiltration of inflammatory cells,and congestion of capillaries; compared with the normal group, IL-4,IL-6,TGF- β 1 in serum increased in rats of the asthma model group and combination group (P<0.05); Penh values increased after stimulation with various concentrations of Mch (P<0.05) .②Rats in the combination group showed symptoms such as chills,curling up with little movement,purple and dark claws,purple and black bruises on the tail,loose stools,and unclean perianal area; compared with the asthma model group,the body mass of rats in the combination group increased (P<0.05); blood glucose,triglycerides,and total cholesterol levels increased (P<0.05),thrombin time shortened (P<0.05),fibrinogen content increased (P<0.05),and whole blood viscosity significantly increased at low,medium,and high shear rates (P<0.05); the indexes were significantly improved after Kechuan Liuwei Mixture treating. Conclusion The asthma rat model with phlegm and blood stasis syndrome can be established through OVA inducing and high-fat diet combined with ice water bath. The model can be evaluated through behavioral observation,indexes detection,and syndrome determination by formulas.