【Abstract】Objective In this study, 2,4-dinitrochlorobenzene (DNCB) was used to induce the establishment of an atopic dermatitis (AD) model in BALB/c homozygous mice to mimic the inflammatory skin complications in patients with clinical malignancies. Methods BALB/c mice were divided into (1) blank control group (NC group), (2) model group (MODEL group), (3) atopic dermatitis group (AD group), and (4) dexamethasone group (DEX group), and after axillary inoculation of the model and dexamethasone groups with S-180 tumor cells, the dorsal skin of the experimental group of mice and the ear were stimulated by using 2,4-Dinitrochlorobenzene given to the experimental group, and a loaded tumor mouse was established. Animal model of atopic dermatitis was established. The body weight of the mice was recorded during the modeling cycle, and after the last administration of the drug, the dorsal skin status of the mice was evaluated, the spleen was taken to calculate the splenic coefficient, the mass difference of the mouse ear pieces was measured to calculate the degree of swelling of the auricle and the rate of inhibition of swelling, and the dorsal skin tissues were taken to do the histopathological examination, and the enzyme-linked immunosorbent assay (ELISA) was used to detect the serum immunoglobulin E (IgE), tumor necrosis factor-alpha (TNF-alpha), interleukin-4(IL-4), and interferon. -4 (IL-4) and interleukin-17 (IL-17) levels. Results Compared with the blank control group, mice in the model and atopic dermatitis groups showed erythematous, papular, scaly, and mossy changes in the skin, decreased body weight, and significantly higher spleen coefficients and auricular swelling. Pathologic findings showed incomplete skin structure, significant thickening of skin thickness, massive inflammatory cell infiltration, and increased number of mast cells. Serum IgE, TNF-α, IL-4 and IL-17 levels were elevated. Compared with the model group, the dexamethasone group showed improvement in various detection indexes. Conclusion 2,4-Dinitrochlorobenzene excitation can successfully establish an animal model of atopic dermatitis in hormonal mice, which is drug-controllable, which provides a useful scientific tool for conducting scientific research related to malignant tumors and skin inflammation.