【】 Objective: To prepare and establish a mouse model of H1N1 influenza wind heat syndrome by combining climate analysis with influenza virus nasal drops. Method: 72 BALB/C mice were randomly divided into 9 groups: control group (Control group), wind heat group (FR-3Day group, FR-5Day group), and model group (1LD-3Day group, 2LD-3Day group, 3LD-3Day group, 1LD-5Day group, 2LD-5Day group, 2LD-5Day group, 3LD-5Day group, with 8 mice in each group. The control group was housed in a normal environment, and the wind heat group and model group were intervened in wind heat conditions for 7 days. In the 8th day, PR8 influenza virus nasal infection was performed, and the control group and wind heat group mice were given equal amounts of physiological saline nasal drops. After virus challenge, each group was housed in a normal environment and samples were taken on the 3rd and 5th days. The appearance of the mice was observed and recorded. The lung index, blood routine, lung tissue pathology, serum IL-6 levels, and virus titers were detected in each group of mice based on their behavioral status, stool, and body temperature. Results:The results were compared with the control group, After 7 days of wind and heat intervention in FR group mice, there were no significant abnormalities in appearance, stool, body temperature, blood routine, and lung tissue pathology; Compared with each group, the appearance, lung index, RBC, HGB, HCT, pathological results, and body temperature of mice progressively worsened with the increase of toxin dosage and the prolongation of time in the Model group; At the same viral dose, NEUTP, LYMPHP, virus titer, and serum IL-6 severity reached their peak on the third day after viral attack, and slightly decreased on the fifth day after viral attack. Conclusion: It is feasible to prepare a mouse model of influenza wind heat syndrome by administering PR8 nasal drops after 7 days of wind heat climate intervention.