Objective To compare the differences and differences of eye drops induced by benzamine chloride solution, hyoscyamine hydrobromide subcutaneous injection, benzamine chloride solution combined with drying oven culture and dry oven culture in mice. Methods 50 SPF C57BL/6J mice were divided into 4 group. Normal group mice were not given any intervention; Mice in the scopolamine group were injected subcutaneously with scopolamine hydrobromide solution daily. Benzalchloramine group mice were given benzalchloramine solution daily. The mice in the drying chamber group were fed in a controlled drying system in a dry environment. The mice in the benzalchloramine combined drying oven group were given benzalchloramine solution daily and fed in a controlled drying system. Body mass, anal temperature, behavioral changes, basic tear secretion test (SIT), tear film rupture time (BUT), corneal fluorescein staining (FLS) and HE staining were observed and recorded before and after modeling. The apoptosis of corneal and lacrimal tissue was detected by TUNEL staining. The expression of NF-kB, IL-1β, TNF-α and other inflammatory factors in lacrimal glands of mice was detected by RT-qPCR. Results Compared with the normal group, the SIT test and BUT in benzalchloramine group after modeling were significantly decreased (P<0.01), the body temperature was increased (P<0.01), the body weight was reduced, the number of apoptosis was increased, and the content of inflammatory factors was increased (P<0.01). Compared with the normal group, after modeling in the scopolamine group, SIT test and BUT were significantly reduced (P<0.01), mice showed a high state of tension and anxiety, and obvious symptoms of dryness in the whole body, dry hair, and small and dry stool. Scores in the field experiment were increased (P<0.01), and OE% and OT% values in the elevated cross maze experiment were decreased (P<0.01). The content of inflammatory factors increased (P<0.05); Compared with normal group, body weight gain decreased and body temperature increased (P<0.01) in benzalchloramine combined drying oven group after modeling, there were significant differences between SIT test and BUT (P<0.01), corneal injury was serious, tear secretion was significantly reduced and inflammatory factor content was increased (P<0.01). After the drying oven group was molded, the difference of each index was small compared with the normal group. Conclusion Eye drops with benzalchloramine solution, subcutaneous injection with scopolamine and combination of benzalchloramine with drying oven culture can induce dry eye model in mice. There is no statistical significance in 28d of drying oven culture alone, and the effect of inducing dry eye model in mice is not obvious. The symptoms of systemic xerosis in scopolamine induced mice model were more severe than ocular surface symptoms, which was more suitable for the construction of sjogren"s syndrome model. The mouse dry eye model induced by benzalchloramine solution combined with drying oven culture method is more stable and durable, with typical clinical symptoms of dry eye eye surface, high operational feasibility and good repeatability, which is an ideal method for dry eye animal model reproduction in teaching, clinical and scientific research experiments.