Abstract:he paper studied the monitoring results for B virus related antibodyand B virus antibody with HSV-1 ( IEA) and B virus ( IFA) :All of the monkeys were B virus antibody negative in IFA,which were Bvirus related antibody negative in IEA.After they had been raised separa-tely for six months, the negative monkeys for B virus related antibodywere detected in IEA, 98.3% of them were still negative for B virusrelated antibody.The paper discussed the feasibility to establish the Bvirus free colony in Rhesus monkeys.