Objective To study the influence of tonifying kidney plus strengthening vital energy recipe on the nonspecific immunity function in H 22 tumor transplanted mice treated with cyclophosphamide(CY). Methods The low immunity model was established in H 22 tumor transplanted mice treated with CY. The activity of natural killer cells and macrophages was measured by LDH release test. The expression of IL-12 mRNA in spleen cells was examined by RT-PCR. Results The recipe could markedly improve the activity of natural killer cells and macrophages in low immunity mice treated with CY and the expression of IL-12 mRNA in spleen cells. Conclusion The recipe can greatly increase the nonspecific immunity function treated with CY in tumor transplanted mice.