Objective To determine susceptibility of adult strain Kunming mouse to rotavirus (RV) infection. Methods Adult strain Kunming mice were experimentally perorally infected by group A rotavirus, human Wa and rhesus SA11. Clinical response was observed and RV antigen in animal stools was detected. Results Apparent diarrhea was observed in mice 2 days post infection and peaked at 4 days; RV antigen could be detected in stool samples collected from infected mice at least from the second to sixth day post infection. Conclusion\ Kunming mouse is susceptible to RV infection and could be used as an adult animal model for evaluation of efficacy of medicinal treatment and vaccine protection to RV infection.