Objective To construct human bone morphogenetic protein-2(hBMP-2 ) expressing eukaryotic vector(pcDNA3-hBMP2) and examine its expression in rabbit bone marrow stromal cells(MSCs).Methods pcDNA3-hBMP2 vector was constructed using gene clone and recombination technique.After being confirmed by enzyme cut,pcDNA3-hBMP2 was transfected into cultured rabbit MSCs in vitro with the help of liposome reagent FuGENE6.The expression of hBMP-2 was determined by in situ hybridization and immunohistochemical analysis.Results The pcDNA3-hBMP2 vector was constructed successfully.The rabbit MSCs transfected by pcDNA3-hBMP2 showed transient and stable expression of BMP2.Conclusion pcDNA3-hBMP2 can express hBMP2 in bone marrow stromal cells.