Objective To establish a method for monitoring H-2 gene in inbred strains of mice.Methods two pairs of specific primers were designed to amplify mice H-2 alleles.Through the specific PCR and polyacryl amide gel electrophoresis(PAGE) to get the H-2 genotype of inbred strains of mice.Results From the PCR products we were able to distinguish the H-2 genotype of different strains.Different inbred strains of mice were distinguished.Conclusion Using this PCR technique,it is possible to monitor the H-2 gene and to supplement the shortage of existing methods.This method is rapid,sensitive and simple to use compared with previous methods.