ObjectiveTo screen markers used in genetic quality detection of inbred strain HAN of Gobiocypris rarus. MethodsLiving donor tranplatation of scales and polyacrylamide gel electrophoresis of isozyme were used for the genetic quality control of HAN inbred strain.ResultsThe survival rate of allotransplanted scales in the inbred strain HAN was significantly higher (80%) than that of 7.4% in the wild population. Six isozymes presumably were encoded by 13 gene loci, 11 of which were found monomorphic in the wild population, and 2 polymorphic loci (est2, est3) with a mean proportion of 15.56%. However, no polymorphic loci was found in F22. In addition, no difference between individuals was found in the inbred stock. ConclusionsAfter successive mating brother and sisiter, inbred stock of G. rarus has reached a high degree of genetic purity. It is feasible to perform genetic quality control of HAN inbred strain of Gobiocypris rarus by scale allotransplantation and gel electrophoresis on Est and Mp. 