Sequence analysis of the complete mitochondrial genome and molecular evolution of Cricetulus griseus
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    Abstract:

    ObjectiveTo obtain the nucleotide sequence of the complete mitochondrial DNA (mtDNA) of Cricetulus griseus to provide molecular data for mitochondrial disease models. MethodsThe complete mitochondrial genome of Cricetulus griseus was sequenced with 27 primers and their PCR amplications, the genomic structural characteristics and gene mapping were analyzed, and to discuss the phylogenetic relationship among 5 rodent species whose complete mitochondrial genome sequences are available in the published databases. ResultsThe complete genome contains 16 283 base pairs and encodes 37 genes,including 13 protein-coding,2 ribosomal RNA and 22 transfer RNA genes. The base composition for the four nucleotides is 33.53 % A, 30.50% T, 12.98 %G and 22.80% C. ConclusionsThe genome of Cricetulus griseus is very similar to that of other vertebrate mitochondrial genomes. Some of the protein-coding regions and the tRNA genes are highly homologous to those of other rodents. The evolution tree built from the homology of 5 kinds of laboratory animals is consistent with the traditional taxonomic results.

    Reference
    [1] 刘田福.中国地鼠山医群体近交系的培育[J].实验动物科学与管理.2003, 20(6): 22-25.
    [2] Papa S.Mitochondrial oxidative phosphorylation changes in the life span molecular aspects and physiopathological implications [J].Biochim Biophys Acta, 6,6(2):87-105.
    [3] 陈丽峰,柳君泽,宋熔,等.氯霉素处理对急性缺氧大鼠脑线粒体氧化呼吸功能变化研究[J].第三军医大学学报,2004,26(18):1611-1614.
    [4] 高文祥,柳君泽,吴利平,等.慢性缺氧对大鼠脑线粒体能量代谢的影响[J].中国病理生理杂志,2000,16(10): 879-882.
    [5] Paradies G, Petrosillo G, Pistolese M, et al.Reactive oxygen species affect mitochondrial electron transport complex I activity through oxidative cardiolipin damage [J].Gene, 2,6(1):135-141.
    [6] 卢圣栋.现代分子生物学实验技术(第二版) [M].北京:中国协和医科大学出版社, 1999.
    [7] 张颖, 聂刘旺, 宋娇莲.缅甸陆龟线粒体全基因组的测序及分析 [J].动物学报, 2007,53(1):151-508.
    [8] Cui P, Ji R, Ding F, et al.A complete mitochondrial genome sequence of the wild two-humped camel (Camelus bactrianus ferus):an evolutionary history of camelidae [J].BMC Genomics,7,8(8):241.
    [9] 毛增辉, 郝家胜, 朱国萍,等.菜粉蝶线粒体基因组的全序列测定和分析 [J].昆虫学报, 0,3(11):1295-1304.
    [10] 季学峰, 吴孝兵, 李艳, 等.尼罗鳄线粒体基因组全序列分析及鳄类系统发生关系的探讨 [J].动物学报, 6,2(4):810-818.
    [11] Liu Y, Cui Z.The complete mitochondrial genome sequence of the cutlassfish Trichiurus japonicus (Perciformes:Trichiuridae):genome characterization and phylogenetic considerations[J].Mar Genomics, 9,2(2):133-142.
    [12] 杨丽萍, 卢迈新, 叶星, 等.尼罗罗非鱼线粒体基因组全序列测定与系统进化分析 [J].中国生物化学与分子生物学报, 0,6(5):484-490.
    [13] Partridge MA, Davidson MM, Hei TK.The complete nucleotide sequence of Chinese hamster (Cricetulus griseus) mitochondrial DNA [J].DNA Seq.7,8 (5):341-346.
    [14] 谭围, 王中铎, 郭昱嵩, 等.孟加拉笛鲷线粒体基因组序列结构及其进化 [J].中国生物化学与分子生物学报, 9,5(3):287-291.
    [15] 杨超, 雷富民, 黄原.地山雀线粒体基因组全序列的测定和分析.动物学研究 [J].2010,31(4):333-344.
    [16] Liu X, Wei F, Li M, et al.Molecular phylogeny and taxonomy of wood mice (genus Apodemus Kaup, 1829) based on complete mtDNA cytochrome b sequences, with emphasis on Chinese species [J].Mol Phylogenet Evol, 4,3(1):1-15.
    [17] Rasmussen AS, Arnason U.Phylogenetic studies of complete mitochondrial DNA molecules place cartilaginous fishes within the tree of bony fishes [J].J Mol.Evol, 9,8(1):118-123.
    [18] Tamura K, Dudley J, Nei M, et al.MEGA4:Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0 [J].Mol Biol Evol, 7,4(8):1596-1599.
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