ObjectiveTo investigate the behavioral changes induced by repeated electrical stimulation of the dura mater surrounding the superior sagittal sinus in conscious adult rats. MethodsTwenty-four male rats were randomly divided into 2 groups. The experimental group was given electrical stimulation (electric current 1 to 2 mA, frequency 20 Hz, sine wave, pulse width 250 μs, lasting 15 minutes, once a day) for 21 days. The changes in body weight, fluid consumption test and open-field test between the two groups in 21 days were compared to evaluate whether depression behavioral changes were induced. Results After 21 days of electrical stimulation, the body weight of the rats of the experimental group was significantly lower than that of the control group (P<0.05). In the experimental group, the number of square crossing and vertical rearing in open-field test was significantly decreased, sucrose solution consumption and preference were significantly reduced, and pure water consumption was significantly increased, all with a significant difference between the two groups (P<0.05). ConclusionsDepression behaviors have been successfully induced by repeated electrical stimulation to dura mater surrounding the superior sagittal sinus for 21 days in conscious rats.