Compared with the rodents such as mice, large laboratory animals (i.e., minipigs and monkeys) have more similarities in size, anatomy, physiology, biochemistry, metabolism and disease pathogenesis with humans. Therefore, they may be widely employed and play important roles in replicating human disease models, studying disease pathogenesis and drug discovery, etc. In recent years, lentivirus-mediated gene delivery approach is increasingly used to produce genetically engineered monkeys and minipigs. Compared with pronuclear microinjection and somatic cell nuclear transfer, lentivirus-mediated gene delivery used to generate transgenic animals enjoys the high transgenic efficacy and simple operation. Therefore, it is quite necessary to set up a technology platform for efficiently producing genetically engineered monkeys and minipigs by lentivirus-mediated gene delivery.