Objective To study the characteristics of mice infected with egg drop syndrome virus (EDSV) strain NE4. Methods Kunming (KM) mice in age of 4[KG-*2]-[KG-*9]6 weeks were artificially infected with 10⁶ TCID₅₀ dose of EDSV strain NE₄, and the negative control mice were inoculated with normal allantoic liquid at the same time. FQ-PCR assay was used to detect the quantity of virus in different tissues. HE and immunohistochemical staining were used to observe the pathological changes and antigen localization. Results The virus inoculation affected somehow the ingestion of mice, but did not cause obvious clinical symptoms. The virus-infected mice produced specific antibody, reaching up to a titer of 2¹². The EDSV titer in the control mice was negative. EDSV was detected in the tissues of most organs such as liver, uterus, kidney, lungs of the mice by FQ-PCR assay, and at 7 days post infection, the mice began to release virus. Immunohistochemistry showed that EDSV was propagated in organs such as liver, uterus, kidney, and lungs of the mice, most notably, in the cytoplasm of uterine glandular epithelial cells and myocytes. No obvious pathological changes were found in tissues of infected mice. Conclusion EDSV strain NE₄ virus can infect KM mice.