Objective TALE-TFs were adopted to provide a new way in detection of the expression result of β-casein gene promoter-interesting gene expression cassettes in mouse fibroblasts. Methods TALE-TFs of eukaryotic expression plasmid and expression cassette with β-casein gene promoter and red fluorescent protein reporter gene were co-nucleofected into mouse fibroblasts by Amaxa nucleofector. Results and Conclusion β-casein gene promoter was activated by artificial TALE-TFs in the mouse fibroblasts. The way is a new expression verification system instead of mammary epithelial cells with fibroblasts.