Objective To determine the interference effect of H.hepaticus infection on the functional characteristics of dendritic cell (DC) surface molecules and immune response in mice. Methods Male BALB/c mice were inoculated with H.hepaticus (ATCC 51450).Murine bone marrow-derived dendritic cells (DC) were isolated and co-cultured which were stimulated by GM-CSF and IL-4 at the fifth month after the last inoculation. Then the DCs were subjected to FACS analysis for surface markers (CD11c, CD40, CD80 and MHCⅡ) detection.On this basis, virus suspension of Newcastle disease virus(NDV) ZJ1 strain was inoculated into the mice. Serum was collected for detection of the NDV antibody titer in serum weekly to explore the difference of antibody titer between the two groups.Results The expression rates of CD40 and MHCⅡ on the mouse DCs in experimental group were higher than that in the control group. The NDV antibody titer of experimental group was slightly lower than that in the control group in the first week. During the 2nd to 5th weeks, the titer was higher than that in the control group, with a very significant difference. In the 6th week, the titer of both the two groups tended to fall.Conclusions H.hepaticus infection can promote bone marrow DC maturation in mice, stimulate the expression rates of MHC Ⅱ and CD40, and enhance the NDV antibody levels.