Objective To establish a pure planarian Dugesia asexual strain in China. Methods The planarian worms were collected from a wild stream, and then made the worm grow after amputation in the lab. To establish an asexual strain through cutting and culturing for the single worms. Results After ten years and more than ten thousand experiments, an asexual pure planarian strain Dugesia ZB-1 originated from Shandong Zibo area was established. It grows stably under laboratory controlled conditions. Conclusions The establishment of Dugesia ZB-1 Priovides a solid foundation for further experiments and promoting planarian research in our country and participation in the field of the international planarian research.