Objective To investigate the feasibility of self-made capacitation liquid for in vitro fertilization of Mongolian gerbils,and to provide a reference for gerbil embryo cryopreservation.Methods In vitro fertilization of Mongolian gerbil was performed with the self-prepared capacitation solution and semen,and the 2-cell embryos of Mongolian gerbils were cultured in vitro using an improved KSOM culture medium.Results The in vitro fertilization rate of gerbils was over 60%,and some gerbil 2-cell embryos could develop further in vitro.Conclusions An in vitro fertilization and embryo development system of Mongolian gerbil has been established,but it needs further optimization.