Analysis of gut microbiota in SD rat model of diabetes mellitus induced by streptozotocin

doi:10.3969/j. issn. 1005 -4847. 2018. 03. 013

Home > Archive>Volume 26, Issue 3, 2018 >349-356. DOI:10.3969/j. issn. 1005 -4847. 2018. 03. 013

Analysis of gut microbiota in SD rat model of diabetes mellitus induced by streptozotocin
DOI:
                        10.3969/j. issn. 1005 -4847. 2018. 03. 013
                    
CSTR:
                        
                    
Author:
                        ZHU HuaZHU Hua
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health; Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center,Peking Union Medical College (PUMC) Beijing 100021,China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
GUO YaxiGUO Yaxi
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health; Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center,Peking Union Medical College (PUMC) Beijing 100021,China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
DU XiaopengDU Xiaopeng
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health; Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center,Peking Union Medical College (PUMC) Beijing 100021,China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
ZHAO WenjieZHAO Wenjie
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health; Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center,Peking Union Medical College (PUMC) Beijing 100021,China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI YanhongLI Yanhong
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health; Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center,Peking Union Medical College (PUMC) Beijing 100021,China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
QIN ChuanQIN Chuan
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health; Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center,Peking Union Medical College (PUMC) Beijing 100021,China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:(Key Laboratory of Human Disease Comparative Medicine,Ministry of Health; Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences (CAMS) & Comparative Medicine Center,Peking Union Medical College (PUMC) Beijing 100021,China)
Clc Number:
Fund Project:CAMS Innovation Fund for Medical Science (No. 2017?12M?2?005)

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Objective To investigate the characteristics of gut microbiota in SD rat model of diabetes mellitus induced by streptozotocin (STZ). Methods Twenty?five male SD rats were randomly divided into control (C) (n =10) and diabetes (M) (n =15) groups. Rats in the group M received intravenous injection of streptozotocin (30 mg/ kg) once per day for 5 consecutive days. Fecal samples were collected and examined for the V3 region of the 16S rDNA gene by Illumina Miseq high?throughput sequencing. The abundance and composition of gut microbiota were analyzed by cluster analysis. Results DNA sequence analysis was successfully performed. The Chao 1 index was lower in the group M than group C ( P < 0.05). The Shannon index was lower and the Simpson index was higher in the group M than group C ( P <0.05). At phylum level, the relative abundance of Proteobacteria, Cyanobacteria, Tenericutes, TM7, and Actinobacteria was lower in the group M than group C ( P < 0. 05). At genus level, 4 weeks after injection,the abundance of Lactobacillus was lower and that of Bacteroidetes was higher in the group M than group C ( P < 0.05). 12 weeks after injection, the relative abundance of Lactobacillus,Bacteroides and Ruminococcus was higher and that of Bifidobacterium was lower in the group M than group C ( P < 0.05). Conclusions This STZ?induced diabetic SD rat model has a low abundance and diversity of gut microbiota. Quantitative analysis of gut microbiota composition in this animal model provides a basic data for the study of relationship between diabetes mellitus and gut microbiota.

Key words:gut microbiota; diabetes mellitus; streptozotocin; high?throughput sequencing

Get Citation

Copy

Article Metrics

Abstract:1590
PDF: 1985
HTML: 0
Cited by: 0

History

Received:March 01,2018
Revised:
Adopted:
Online: July 20,2018
Published:

Home

About this journal

Editorial Board

Info for Author

Contact Us

中文版

Get Citation

Related Videos

Share

Article Metrics

History

Article QR Code