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Home > Archive>Volume 27, Issue 3, 2019 >298-304. DOI:10. 3969 / j.issn.1005-4847. 2019. 03. 005
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Effects of X-ray whole body irradiation on the hematopoietic immune system in KKAy mice with type 2 diabetes mellitus
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(1. Institute of Laboratory Animal Science, Chinese Academy of Medical Science & Comparative Medicine Center,Peking Union Medical College, Key Laboratory of Human Disease Comparative Medicine,Ministry of Health,Beijing Engineering Research Center for Laboratory Animal Models of Human Critical Diseases, Beijing 100021, China.2. Beijing Chemical Occupational Disease Prevention and Treatment Institute, Beijing 100176)

Clc Number:

Q95-33

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    Abstract:

    Objective To explore the damaging effect of X-ray whole body irradiation on hematopoiesis andimmune functions in KKAy mice with type 2 diabetes mellitus. Methods KKAy mice were exposed to 4 Gy total bodyirradiation (TBI) ,and compared with C57BL/6J mice. All mice were euthanized for routine analysis of peripheral blood,hematopoietic progenitor cells (HPC), hematopoietic stem cells (HSC), and long-term hematopoietic stem cells (LTHSC)frequencies and the percentages of splenic lymphocytes and thymic lymphocytes. The function of HPCs was measuredby colony-forming unit-granulocyte and macrophage (CFU-GM) assay. Results The frequency of HSCs and LT-HSCs inKKAy mice was lower than that in C57 mice. After 4 Gy whole body irradiation, the percentages of WBCs, RBCs, PLTs,HG B and LYM% in peripheral blood of KKAy mice were decreased by 68. 42%, 12. 17%, 8. 78%, 30. 12%, and70. 84%, respectively. The percentages of HPCs, HSCs and LT-HSCs in bone marrow were decreased by 34. 02%,29. 49%, 35. 74%, respectively. The proportions of splenic B and T cells were decreased by 57. 85% and 58. 81%,respectively. The proportion of thymic CD4+ / CD8+ cells was decreased by 51. 7%. The HPC function (CFU-GM) was alsoimpaired. The decrease levels of bone marrow HSC, LT-HSC, peripheral blood RBC and HGB in KKAy mice weresignificantly lower than those in C57 mice.Conclusions Whole body irradiation by 4 Gy X-rays impairs the hematopoiesis and immune functions, and KKAy mice might show a greater tolerance to ionizing radiation than C57 mice.

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  • Received:December 28,2018
  • Online: July 09,2019
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