Cloning and characterization of presenilin 1 in the Chinese tree shrew

doi:10. 3969 / j.issn.1005-4847. 2019. 04. 006

Home > Archive>Volume 27, Issue 4, 2019 >456-465. DOI:10. 3969 / j.issn.1005-4847. 2019. 04. 006

Cloning and characterization of presenilin 1 in the Chinese tree shrew
DOI:
                        10. 3969 / j.issn.1005-4847. 2019. 04. 006
                    
CSTR:
                        
                    
Author:
                        LI Mingxue1LI Mingxue
Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
WANG Wenguang1WANG Wenguang
Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI Na1LI Na
Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
KUANG Dexuan1KUANG Dexuan
Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
TONG Pinfen1TONG Pinfen
Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
HUANG XinHUANG Xin
1. Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China. 2. Kunming Medical University, Kunming 650500
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI Xiaohui1LI Xiaohui
Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
SUN Xiaomei1SUN Xiaomei
Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LU Caixia1LU Caixia
Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China.
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:(1. Tree Shrew Germplasm Resource Center, Institute of Medical Biology, Chinese Academy of Medical Sciences (CAMS) &Peking Union Medical College(PUMC), Kunming 650118, China. 2. Kunming Medical University, Kunming 650500)
Clc Number:Q95-33
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Objective To get the full-length presenilin-1 (PSEN1) cDNA encoding sequence and analyze itsmolecular characteristics. Methods Full-length PSEN1 cDNA was cloned from total RNA from brain tissue of tree shrewby RT-PCR and RACE-PCR. The molecular characteristics were compared with PSEN1 in other mammals and evaluatedusing biology softwares such as DNAMAN, MEGA and others. Quantitative reverse transcription PCR and western blotassays were used to examine the mRNA and protein expression pattern of PSEN1 in various tissues of tree shrew. ResultsThe open reading frame sequence of PSEN1 was 1128 bp and encoded 375 amino acids. We constructed a phylogeneticfamily tree and compared their PSEN1 amino acid sequences. Tree shrew PSEN1 was closer to humans and non-humanprimates than mouse and rat. The expression of PSEN1 mRNA and protein was obviously higher in the brain than that ofother organ tissues. Conclusions We successfully cloned the PSEN1 gene in tree shrew and provided comparative analysiswith other species. These findings provide a theoretical basis for further study on the function of this gene and the establishment of animal models of Alzheimer’s disease (AD).

Key words:tree shrew; PSEN1; cloning; phylogenetic analysis

Get Citation

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:April 12,2019
Revised:
Adopted:
Online: September 04,2019
Published:

Home

About this journal

Editorial Board

Info for Author

Contact Us

中文版

Get Citation

Related Videos

Share

Article Metrics

History

Article QR Code