Anti-atherosclerotic and cardioprotective effects of perilla seed extract on ApoE -/ - mice
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(1.Guangdong Pharmaceutical University, School of Life Sciences and Biopharmaceuticals, the Vascular Biology Institute, Guangzhou 510006, China. 2. Guangdong Laboratory Animals Monitoring Institute, Guangdong Key Laboratory for Laboratory Animals, Guangzhou 510633)

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    Abstract:

    Objective To study the role and molecular mechanism of perilla seed oil extract (PSO) in reducing arterial plaque formation and providing cardioprotection in ApoE -/ - mice. Methods Three-month-old ApoE -/ - mice were randomly divided into ApoE -/ -(no drug treatment), low-dose PSO, high-dose PSO and atorvastatin groups. B6 mice of the same age were used as controls (WT group); they were fed a regular diet. After 8 weeks of treatment, biochemical tests, H&E staining, echocardiography, qPCR, ATP detection and Pro-Q Diamond staining were performed. Results Compared with the WT group, ApoE -/ - mice showed abnormal cardiac structure and function. After PSO treatment, the plaque area was reduced, and cardiac structure and function improved. mRNA expression of ANP, BNP and β-MHC was downregulated, mRNA expression of PPARα and PGC-1α was up-regulated, and the intracellular content of ATP and the phosphorylation level of TnI were increased. Conclusions PSO inhibits arterial plaque formation and maintains normal cardiac structure and function in ApoE -/ - mice. It may regulate the PPARα/ PCG-1α/ NRF-1 axis to increase ATP and myofilament phosphorylation, improve energy metabolism, and exert cardioprotective action.

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History
  • Received:June 19,2019
  • Online: January 07,2020
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