Study on the effects of imatinib on the development of blood vessels and nerves in zebrafish

doi:10. 3969 / j.issn.1005-4847. 2020. 03. 001

Home > Archive>Volume 28, Issue 3, 2020 >289-296. DOI:10. 3969 / j.issn.1005-4847. 2020. 03. 001

Study on the effects of imatinib on the development of blood vessels and nerves in zebrafish
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                        10. 3969 / j.issn.1005-4847. 2020. 03. 001
                    
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                        BAO YiqingBAO Yiqing
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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CHEN SiCHEN Si
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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HONG RuishengHONG Ruisheng
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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ZHU YiZHU Yi
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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GAO MinGAO Min
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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LI LeleLI Lele
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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ZHANG XuZHANG Xu
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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WANG XinWANG Xin
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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LIU DongLIU Dong
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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SHI YunweiSHI Yunwei
Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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Affiliation:Key Laboratory of Neuroregeneration, School of Life Science, Medical School, Nantong University, Nantong 226001, China
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Abstract:

Objective Using the advantages of zebrafish embryo transparency for live imaging, the role of imatinib in the development of blood vessels and nerves in zebrafish was studied. Methods First, we investigated the safe concentration range of imatinib for zebrafish in the blood vessel and neuron development stage using wild type zebrafish embryos. Within the safe concentration range, transgenic zebrafish embryos with a labeled vascular system were treated with imatinib. Moreover, endothelial cell and neuron co-labeled zebrafish were treated with imatinib to study the specificity for angiogenesis inhibition. The molecular mechanism of imatinib-inhibited angiogenesis was investigated. Results Imatinib concentration below 100 μg / mL was not lethal to zebrafish embryos. In this concentration, imatinib significantly inhibited the development of zebrafish intersegmental vessels (ISVs), including the process of ISV lumenization and the proliferation of endothelial cells. Additionally, imatinib had no significant effect on motor neurons and neuronal precursor cells. Moreover, gene expression analysis showed that imatinib was able to significantly inhibit vegfr2 and pdgfrα mRNA expression. Conclusions Imatinib significantly inhibited angiogenesis in zebrafish embryos by regulating VEGF and PDGFR signals.

Key words:imatinib; angiogenesis; endothelial cell; neuron; zebrafish

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Received:December 24,2019
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Online: July 03,2020
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