Metabolic analysis of human flora-associated mice with coronary heart disease using liquid chromatography-mass spectrometry

doi:10. 3969 / j.issn.1005-4847. 2020. 03. 005

Home > Archive>Volume 28, Issue 3, 2020 >323-329. DOI:10. 3969 / j.issn.1005-4847. 2020. 03. 005

Metabolic analysis of human flora-associated mice with coronary heart disease using liquid chromatography-mass spectrometry
DOI:
                        10. 3969 / j.issn.1005-4847. 2020. 03. 005
                    
CSTR:
                        
                    
Author:
                        ZHU HuaZHU Hua
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) Comparative Medical Center, Peking Union Medical College (PUMC), Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
GUO YaxiGUO Yaxi
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) Comparative Medical Center, Peking Union Medical College (PUMC), Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
DU XiaopengDU Xiaopeng
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) Comparative Medical Center, Peking Union Medical College (PUMC), Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI ZhuoLI Zhuo
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) Comparative Medical Center, Peking Union Medical College (PUMC), Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
SU LeiSU Lei
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) Comparative Medical Center, Peking Union Medical College (PUMC), Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
QIN ChuanQIN Chuan
Key Laboratory of Human Disease Comparative Medicine,Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) Comparative Medical Center, Peking Union Medical College (PUMC), Beijing 100021, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:Key Laboratory of Human Disease Comparative Medicine,Ministry of Health, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences (CAMS) Comparative Medical Center, Peking Union Medical College (PUMC), Beijing 100021, China
Clc Number:
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Objective To use liquid chromatography-mass spectrometry based plasma metabonomics to study the characteristics of human flora-associated (HFA) mice with coronary heart disease. Methods Twenty-eight female germ free C57BL/ 6J mice were divided into healthy control and coronary heart disease (CAD) groups. Each mouse was orally inoculated with a stool suspension from healthy people or patients with CAD to establish the HFA mouse models. At 6 and 10 weeks after inoculation, plasma metabolites were studied and PCA and PLS-DA statistical method were used to identify the characteristic metabolites. Results The PCA, PLS-DA and variable importance in projection analysis identified 30 characteristic metabolites present at 6 and 10 weeks after inoculation; structural identification found that L-carnitine, phenylpyruvic acid, and 1-naphthol, 2-naphthol were significantly higher in the CAD group compared with the control group. These potential biomarkers were related to the bile acid pathway and glycine, serine, threonine pathway. Conclusions The metabolism disorder in HFA mice appears similar to that of patients with CAD.

Key words:coronary heart disease; human flora associated animal; LC-MS; metabolomics

Get Citation

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:December 11,2019
Revised:
Adopted:
Online: July 03,2020
Published:

Home

About this journal

Editorial Board

Info for Author

Contact Us

中文版

Get Citation

Related Videos

Share

Article Metrics

History

Article QR Code