Ultrasound-stimulated microbubble technology is becoming increasingly more common in the clinical setting. Its uses mainly include targeted drug delivery, enhanced immunotherapy, gene transfection, opening of the bloodbrain barrier, damaging tumor blood vessels, and assisting in thrombus ablation. In this paper, the preparation of ultrasonic microbubbles and the application of ultrasonic microbubbles in zoopery are summarized with the aim of providing a theoretical reference for the practical application of ultrasonic microbubbles in clinical practice.