DENG Shuwei
1. Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China. 2. Beijing Infectious Diseases Research Center, Beijing 100015. 3. National Medical Center for Infectious Diseases, Beijing 100015.DU Chunjing
4. Department of Critical Care Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015ZHANG Yue
1. Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China. 2. Beijing Infectious Diseases Research Center, Beijing 100015. 3. National Medical Center for Infectious Diseases, Beijing 100015.ZHOU Yanxi
1. Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China. 2. Beijing Infectious Diseases Research Center, Beijing 100015. 3. National Medical Center for Infectious Diseases, Beijing 100015.ZHANG Liang
5. Pathology Department Beijing Ditan Hospital, Capital Medical University, Beijing 100015QIN Qiushi
6. Peking University Ditan Teaching Hospital, Beijing 100015ZHU Liuluan
1. Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China. 2. Beijing Infectious Diseases Research Center, Beijing 100015. 3. National Medical Center for Infectious Diseases, Beijing 100015.6. Peking University Ditan Teaching Hospital, Beijing 1000151. Beijing Key Laboratory of Emerging Infectious Diseases, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China. 2. Beijing Infectious Diseases Research Center, Beijing 100015. 3. National Medical Center for Infectious Diseases, Beijing 100015. 4. Department of Critical Care Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015. 5. Pathology Department Beijing Ditan Hospital, Capital Medical University, Beijing 100015. 6. Peking University Ditan Teaching Hospital, Beijing 100015
Objective To construct a mouse model of Pneumocystis pneumonia ( PCP) and evaluate the immunological characteristics of the model mice. Methods A PCP model was established by endotracheal infusion of Pneumocystis murine into severe combined immune deficiency mice. The etiology was identified by quantitative real-time PCR and silver hexaammonium staining of lung tissue. The degree of lung tissue injury was assessed by HE staining, and immunological evaluation was performed by immunofluorescence staining and flow cytometry. Results After six weeks of P. murina infection, there were significantly more lung tissue rRNA copies from P. murina in the experimental group than the sham group. P. murina trophozoites and cysts were detected in lung tissue sections from mice in the experimental group by silver hexaammonium staining. HE staining showed the alveolar walls of lung tissues in the experimental group were thickened, the lung interstitium was widened, and a large number ofGr-1+ neutrophils andCD68+ macrophages had infiltrated the lungs. The levels of myeloperoxidase, a marker of neutrophil activation, and nitric oxide synthase, a marker of macrophage activation, were significantly increased. Flow cytometry showed that the number and proportion of neutrophils and monocytes in the lungs and alveolar lavage fluid were significantly higher in the experimental group than the control and sham groups. Conclusions A stable PCP mouse model was established by endotracheal infusion of P. murina into SCID mice, which showed immunological characteristics similar to clinical signs.
