Objective A visual metastasis model of hepatoma cells and cancer-associated fibroblasts (CAFs) was established using zebrafish to provide a preclinical model for CAFs-mediated mechanism research and therapeutic efficacy evaluation of patients with tumor metastasis. Methods After establishing a co-culture system, the proliferation and migration of Huh7 cells were assessed by CCK8 and Transwell assays, respectively. Huh7 cells and CAFs labeled with different fluorescent dyes were injected into the perivitelline space of zebrafish to establish a visual metastasis model. The effects of CAFs on Huh7 cell migration and Huh7 cell binding to CAFs in blood vessels were observed. Results The proliferation and migration of Huh7 cells co-cultured with CAFs were increased significantly. A visual metastasis model of hepatoma cells and CAFs in zebrafish was established successfully. The migration and binding rate of Huh7 cells co-injected with CAFs were increased significantly. Conclusions CAFs promote migration of hepatoma cells, and most hepatoma cells are tightly bound to CAFs in distal metastases. Therefore, fibroblasts of solid tumors can be targeted to block their support of tumors and provide a new strategy for cancer therapy.