Objective To compare three kinds of yang?deficiency STC rat models by a combination of disease and syndrome evaluation to provide an ideal experimental animal model for TCM prevention and treatment of this disease. Methods Sixty?four SD rats were divided into blank and model groups. The rats were treated with loperamide hydrochloride combined with 0℃ ice water by gavage (M?1 group), loperamide hydrochloride combined with dahuang decoction (M?2 group), and white vinegar and 0℃ ice water by gavage alternately + loperamide hydrochloride by gavage (M?3 group) (16 rats in each group) to establish the three yang?deficiency slow transit constipation rat models. The blank group (n= 16) was administered the same volume of distilled water by gavage. Model groups were established by the corresponding method. The general condition of rats was observed during modeling. Rectal temperature, food intake, voluntary activity, stool Bristol score, and water content were measured at fixed times every week. After establishing the model, the discharge time of the first black stool and the intestinal propulsion rate were measured. Rat colon pathological changes were observed by HE staining. Colon mucous changes were assessed by PAS staining. Colon 5?HT, VIP, and SP were analyzed by ELISA and IHC. Results Blank group rats before and after building without exception. After modeling, rats in each model group showed listlessness, lethargy, and crouching. Some rats died during modeling. Mortality was in the order of M?3 group > M?2 group > M?1 group. Compared with the blank group, the anal temperature, food intake, and voluntary activity of each model group showed a downward trend during modeling. The model groups were compared with the blank group as follows: M?1 group: building 3 weeks of food intake, autonomous activity was obviously decreased (P<0.01), building 4 weeks of rectal temperature and fecal water content were significantly decreased (P< 0.01), building 5 weeks of Bristol stool scale was significantly decreased (P< 0.01). M?2 Group: building 3 weeks of rectal temperature, food intake, and autonomous activity were significantly decreased (P< 0.01), building 6 weeks of the Bristol stool scale and fecal water content were significantly decreased (P< 0.01). M?3 Group: building 1 week of autonomous activity was decreased significantly (P< 0.01), building 2 weeks of food intake significantly decreased (P< 0.01), building 4 weeks of rectal temperature, Bristol stool scale, and fecal water content were significantly decreased (P< 0.01). HE staining showed no obvious pathological changes in the colon of rats in each group after modeling. PAS staining showed that the mucus layer thickness in the colon of rats in each model group was significantly reduced (M?3 < M?1 < M?2). Compared with the blank group, the first black stool excretion time in the model groups was increased, and the intestinal propulsion rate, and 5?HT and SP in the colon were significantly decreased (P< 0.01). VIP in the colon of M?1 group was significantly increased (P< 0.01). Conclusions The STC model of yang?deficiency can be established by the three modeling method. The rat model established by intragastric administration of loperamide hydrochloride combined with 0℃ ice water has the advantages of low mortality, good safety, stable yang?deficiency syndrome, obvious changes in stool characteristics, and significant pathological changes in intestinal motility. It is an ideal model to study the STC pathogenesis of yang?deficiency and the effect of traditional Chinese medicines.