+Advanced Search
Home > Archive>Volume 31, Issue 6, 2023 >706-714. DOI:10. 3969 / j.issn.1005-4847. 2023. 06. 002
PDF HTML XML Export Cite reminder
Risk factors of cognitive impairment influence histopathological morphology and iron content in hypertensive rats
Author:
Affiliation:

1. Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. 2. Institute of Cardiovascular Disease of China Academy of Chinese Medicine Science, Beijing 100091. 3. Institute of Geriatrics of China Academy of Chinese Medical Sciences, Beijing 100091. 4. Graduate School, China Academy of Chinese Medical Sciences, Beijing 100700. 5. Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102. 6. Beijing University of Chinese Medicine, Beijing 100029

  • Article
    • | |
  • Metrics
    • |
  • Reference
    • | |
  • Cited by
    • | |
  • Comments
    Abstract:

    Objective To observe changes on pathological morphology and iron content in brain tissue of spontaneously hypertensive rats (SHR) with multiple risk factors (high fat, salt, and carbohydrate diets). Methods Fourteen-week-old SPF grade, 8 rats in each group, normal control group(share the same genetic background, the WKY group). SHR rats were randomly divided into four groups: control group (SHRs group), high fat diet group (HFAT-SHRs group), high salt diet group (HSAIL-SHRs group), and high glucose and fat diet group (DM-SHRs group was established by intraperitoneal injection of 1% streptozotocin. And at the same time and blood glucose content was kept above 11. 1 mmol/ L was regarded to be the diabetes model standard). WKY group and SHRs group was fed an ordinary diet, and the model groups were fed high fat, salt, or sugar diets. The treatments were carried out for 32 weeks. Changes in iron content in serum and brain tissue were measured, pathological morphology and iron content (Prussian blue staining) of brain tissue were observed under a light microscope, and the levels of inflammatory factors, and cholinergic and β-AP contents were measured by ELISA. Results Compared with the WKY group, brain cells in each model group showed a disordered arrangement, nuclear pyrosis, and decreased or absent Nissl bodies. Iron staining showed expansion of the iron area, especially in the DM-SHRs group. ACh and AChE contents in the SHRs group and three model groups were decreased, while IL-1β and IL-6 contents were increased (P< 0. 05, P< 0. 05), and iron content in serum and brain tissue was increased (P< 0. 05, P< 0. 05). There was no statistical significance within groups (P> 0. 05). Conclusions High fat, salt, and sugar are factors that change the morphology of brain cells, promote iron deposition, and inhibit endogenous antioxidant activity. Iron overload may lead to cognitive impairment by hypertensive brain damage.

    Reference
    Related
    Cited by
Get Citation

Copy
Related Videos

Share
Article Metrics
  • Abstract:422
  • PDF: 815
  • HTML: 0
  • Cited by: 0
History
  • Received:December 26,2022
  • Online: September 06,2023
Article QR Code
You are the first Visitors
® 2025 All Rights Reserved Governing Body：中国科学技术协会 Organizer：中国实验动物学会 中国医学科学院医学实验动物研究所
Address：5 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China Postcode：100021 Phone：010-67779337 E-mail:b67769337@126.com
Supported by:Beijing E-Tiller Technology Development Co., Ltd.