Objective To investigate the effect of μ opioid receptor (μRs) blockers on the exercise ability and behavior of learned helplessness mice. Methods Mice were randomly divided into a learning-regression group, conjugate group, and control group. Before the mice learned to avoid the electric shock stimulus experiment, the μRs blocker β-FNA or normal saline (Sal) was used to predict the treatment. Mouse movement distances in the open field experiment were compared by T test to determine the effect of the intervention on the mobility of mice. The helplessness behavior of mice after blocking μRs was investigated by shuttling box, forced swimming, elevated cross maze, and open field experiments. Results There was no significant difference in exercise distance between the two groups compared with the control group. There was no difference in the time of electric shock between the two treatment groups during the three-day learning period. There was a significant difference between the two treatment groups for the total number of times touching the nasal contact devices on the second and third days (P< 0. 001). Conclusions The μRs blocker had no effect on the exercise ability of learned helplessness mice, and μRs played an important role in the formation of aversive behavior in mice.