Bronchoalveolar lavage is an important technique to study respiratory diseases and has been widely used in disease research. Currently, the procedure of bronchoalveolar lavage in patients has been gradually standardized. However, in mice, as an important model animal for studying lung diseases, standard collection and testing procedures for bronchoalveolar lavage fluid are needed. An unstandardized procedure can hinder the promotion and application of this technique in research and affects the accuracy and reliability of experimental result. This review presents a systematic summary of domestic and international studies of bronchoalveolar lavage method for mice to provide a reference and guidance for researchers applying and establishing a standardized lavage procedure.