Model establishment and evaluation of chronic obstructive pulmonary disease complicated with chronic kidney disease in rats

doi:10. 3969 / j.issn.1005-4847. 2023. 10. 004

Home > Archive>Volume 31, Issue 10, 2023 >1271-1277. DOI:10. 3969 / j.issn.1005-4847. 2023. 10. 004

Model establishment and evaluation of chronic obstructive pulmonary disease complicated with chronic kidney disease in rats
DOI:
                        10. 3969 / j.issn.1005-4847. 2023. 10. 004
                    
CSTR:
                        
                    
Author:
                        FAN ZhengyuanFAN Zhengyuan
1.the First Clinic School, Henan University of Chinese Medicine, Zhengzhou 450046, China. 2. Chinese Medicine Pharmacology (Respiratory) Laboratory, Henan Key Laboratory of Traditional Chinese Medicine for the Prevention and Treatment of Respiratory Diseases, the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI YaLI Ya
2.Chinese Medicine Pharmacology (Respiratory) Laboratory, Henan Key Laboratory of Traditional Chinese Medicine for the Prevention and Treatment of Respiratory Diseases, the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000. 3. Respiratory Department of the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI SuyunLI Suyun
3.Respiratory Department of the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000. 4. Co-Construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of China, Henan University of Chinese Medicine, Zhengzhou 450046
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI GaofengLI Gaofeng
1.the First Clinic School, Henan University of Chinese Medicine, Zhengzhou 450046, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site
LI JingmeiLI Jingmei
1.the First Clinic School, Henan University of Chinese Medicine, Zhengzhou 450046, China
Find this author on All Journals
Find this author on BaiDu
Search for this author on this site

                    
Affiliation:1.the First Clinic School, Henan University of Chinese Medicine, Zhengzhou 450046, China. 2. Chinese Medicine Pharmacology (Respiratory) Laboratory, Henan Key Laboratory of Traditional Chinese Medicine for the Prevention and Treatment of Respiratory Diseases, the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000. 3. Respiratory Department of the First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450000. 4. Co-Construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan & Education Ministry of China, Henan University of Chinese Medicine, Zhengzhou 450046
Clc Number:
Fund Project:

Article

Figures

Metrics

Reference

Cited by

Materials

Comments

Abstract:

Objective To establish and evaluate a model of chronic obstructive pulmonary disease (COPD) combined with chronic kidney disease (CKD) in rats. Methods Forty SPF grade SD rats were randomly divided into Control group, COPD group, CKD group, and COPD combined with CKD model (COPD + CKD) group, with 10 rats in each group. The COPD rat model was prepared by cigarette smoke exposure combined with the bacterial drip method, the CKD rat model was established by adenine induction, and the COPD combined with CKD rat model was prepared by both method. Results After successful modeling, lung function indexes incluidng forced vital capacity ( FVC), forced expiratory volume in 0. 1 s (FEV_{0. 1} ), and FEV_{0. 1} / FVC were significantly reduced in the COPD + CKD group (P< 0. 01), and lung histopathology showed emphysematous changes with alveolar wall fracture and fusion as well as inflammatory cell infiltration. Serum Cr, BUN, and 24 h urine protein were significantly increased (P< 0. 01). Renal histopathology showed glomerular mesentery proliferation, basement membrane thickening, tubular dilatation, and interstitial fibrosis. The ultrastructure showed that glomerular capillary loops were partially closed, foot processes were fused, renal tubule mitochondria were fused and disintegrated, and lysosome was increased. Serum IL-6, IL-13, IL-1β, and TGF-β1 levels were significantly increased (P< 0. 01) and were significantly higher than those in single model groups (P< 0. 01). Conclusions Cigarette smoke exposure combined with bacterial infection and 2. 5% adenine induction successfully establishes a model of COPD complicated with CKD in rats, and the inflammatory response might play a major role in the process of COPD complicated with CKD.

Key words:chronic obstructive pulmonary disease; chronic kidney disease; rat model

Get Citation

Copy

Article Metrics

Abstract:
PDF:
HTML:
Cited by:

History

Received:June 29,2023
Revised:
Adopted:
Online: December 29,2023
Published:

Home

About this journal

Editorial Board

Info for Author

Contact Us

中文版

Get Citation

Related Videos

Share

Article Metrics

History

Article QR Code