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Home > Archive>Volume 31, Issue 10, 2023 >1304-1313. DOI:10. 3969 / j.issn.1005-4847. 2023. 10. 008
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Establishment of HPLC-MS / MS quantitative analysis method and pharmacokinetic characteristics of Flavokawain B of plasma in mice
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1.Beijing Key Laboratory of TCM Basic Research on Prevention and Treatment of Major Disease, Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China. 2. Institute of Chinese Materia Medica, China Academy of Chinese Medicine Sciences, Beijing 100700

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    Abstract:

    Objective To create a high-performance liquid chromatography-tandem mass spectrometry (HPLCMS/MS) method for the quantitative analysis of Flavokawain B in plasma, and to investigate its pharmacokinetic characteristics in mice. Methods Flavokawain B was administered to KM mice via intravenous injection (20 mg/ kg), intraperitoneal injection (20, 40, and 60 mg/ kg), and intragastric(200, 400, and 600 mg/ kg) routes. Blood was drawn from the venous plexus of the eye fundus at 0, 5, 30 min and 1, 2, 4, 6, 8, 12, 16, and 24 h and then the plasma was separated. Flavokawain B levels in the plasma samples were analyzed by HPLC-MS/ MS, and its pharmacokinetic parameters and bioavailability following the different routes of administration were calculated. Results Flavokawain B showed a good linear relationship in plasma (r = 0. 9995), with a low limit of quantification of 0. 2 ng/ mL, accuracy -8. 50% ~ 12. 50%, precision 1. 73% ~ 12. 03%, and no obvious matrix effect (88. 68% ~ 102. 04%). HPLC-MS/ MS was verified as a suitable method for the quantitative evaluation of Flavokawain B in mice plasma. Flavokawain B showed rapid absorption in vivo, with a peak of plasma concentrations at 0. 083 h after intraperitoneal or intragastric administration. Flavokawain B showed good linear pharmacokinetics following intraperitoneal injection of 20 ~ 60 mg/ kg and intragastric administration of 200 ~ 600 mg/ kg. The absolute bioavailability of Flavokawain B was 53. 29% following intraperitoneal injection and 1. 38% following intragastric administration. Conclusions The current quantitative HPLC-MS/ MS technique provides a straightforward, precise, and sensitive method for investigating the pharmacokinetics of Flavokawain B in mice.

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  • Received:March 03,2023
  • Online: December 29,2023
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