The construction of experimental animal models plays an important supporting role in research into the mechanisms of action of Chinese medicines. There have been increasing reports of the construction and evaluation of animal models of spleen deficiency; however, the construction method have involved different standards and there has been insufficient objectification of the evaluation indexes. In this review, we summarize the construction and evaluation method of animal models of spleen deficiency from the aspects of animal selection, model establishment, macroscopic characterization, behavioral experiments, and Objective indexes of spleen deficiency, with a view to providing theoretical guidance for the construction of experimental animal models of spleen deficiency and references for the selection of animal model platforms for spleen deficiency.