Objective To assess the sweat secretion of Yin-deficient ovariectomized rats and investigate the changes in biochemical indexes. Methods Eighteen SD female rats were randomly divided into a sham operation group,model group, and positive control group of six rats each. The rats in the sham operation group underwent a sham operation,and those in the model group and positive control group underwent bilateral ovariectomy. L-Thyroxine ( 92 mg / kg) was given once a day for 7 consecutive days starting on the 7th postoperative day to establish a Yin-deficient ovariectomized model. The rats in the positive control group were orally administered Qinggu San Tang ( 7. 3 g / kg) once a day, while those in the sham operation group and model group were orally administered an equal amount of distilled water once a day for a total of 14 days. Sweat secretion from the plantar region of the foot was measured using the Wada-Takagaki reagent coloring method. At the end of the experiment, blood was taken from the abdominal aorta and the tissue of the paw pads was separated. The serum levels cyclic adenosine monophosphate ( cAMP), cyclic guanosine monophosphate ( cGMP),luteinizing hormone ( LH), gonadotropin-releasing hormone ( GnRH), and estradiol ( E₂ ) were measured by enzymelinked immunosorbent assay. Western Blot was used to determine the expression levels of M₃R, β₂AR, and aquaporin-5(AQP₅ ) in the paw pad. Results The three main findings of this study were as follows. (1) Compared with the rats in the sham operation group, those in the model group were more irritable and aggressive, and their body weights decreased while their average temperature and sweat secretion significantly increased. (2) Serum cAMP level and cAMP / cGMP ratio increased, the LH and GnRH levels significantly increased, and the E₂ level decreased. (3) M₃R expression was downregulated and β₂AR and AQP₅ expression was up-regulated in the paw pads of the rats. After 2 weeks of positive control treatment, the serum cAMP level and cAMP / cGMP ratio significantly decreased and the LH and GnRH levels decreased;however, no statistically significant difference was observed in the serum E₂ level. The expression levels of M₃R were increased-regulated in paw pads of the rats, and reduced expression of β₂AR and AQP₅ . Conclusions Sweat secretion significantly increased in this “combined disease and evidence” model of perimenopausal syndrome kidney yin deficiency established by desiccation combined with thyroxine. The underlying mechanism may be related to the changes in cGMP,cAMP, and key proteins M₃R, β₂AR, and AQP₅ in sweat glands that regulate sweat secretion.