Objective To establish and optimize an animal model of atopic march (AM), skin and lung tissue sensitization under single or combined modeling of ovalbumins (OVA)and calcipotriol (MC903)was compared. Methods 40 SPF BALB/c mice aged 6 ~ 8 weeks were randomly divided into a control group, model group A, model group B and model group C. The 3 of AM models were established with MC903, MC903 + OVA and OVA, respectively, through skin sensitization ( twice) and respiratory sensitization (once). Skin sensitization severity scoring system, immunohistochemistry, and enzyme-linked adsorption assay were used to compare skin lesion morphology, skin or lung histopathology, and immunophenotype. Results Compared to OVA or MC903 modeling alone, MC903 + OVA modeled mice showed more significant changes in skin morphology, a higher score for skin sensitization severity, more severe skin and airway inflammatory cell infiltration, and more significant changes in the expression of the related inflammatory factors thymic stromal lymphopoietin (TSLP), interleukin 4 (IL-4), IL-13 and IL-10 (P<0.05). Conclusions An AM animal model optimized by MC903 combined with OVA was successfully constructed that provides a good method ological basis for AM mechanism research.