Objective To compare the serological and pathological characteristics of 3 nonalcoholic steatohepatitis (NASH) models: high-fat diet (HFD) with carbon tetrachloride (CCl₄ ) injection, methionine and choline deficient diet (MCD), and Aymlin liver NASH (AMLN) diet-induced NASH models. Methods 3 NASH models were established by feeding mice an HFD with CCl₄ injection for 10 weeks, MCD for 8 weeks and NASH for 26 weeks. After feeding, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose (GLU), liver triglyceride (TG), total cholesterol (TC), and malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were measured. Insulin levels were measured by enzyme-linked immunosorbent assay (ELISA) and the homeostasis model assessment of insulin resistant (HOMA-IR) index was calculated. Hematoxylineosin (HE), Sirius red, and oil red staining were used to indicate pathological changes to the liver. The NAS score was used to grade the pathology. Results Compared to each normal control (NC) group mice, all mice in the 3 model groups had an obvious increase in serum transaminase and liver TG, TC, MDA levels and SOD activity. The levels of serum FINS, GLU and the HOMA-IR index were significantly increased in the AMLN and CCl₄ + HFD model groups but decreased in the MCD model group. According to the HE, oil red staining and NAS score, mice in all 3 groups had NASH phenotypic changes. Liver collagen deposition was most obvious in mice in theCCl₄ + HFD model group. Liver lipid droplets were most abundant in the AMLN model group. Conclusions All the above 3 animal models can stably simulate the serological and pathological changes of NASH in human. The AMLN model can simulate the progress and mechanism of the disease, as well as systemic metabolic disorders such as insulin resistance and oxidative stress. However, it is time-consuming and the fibrosis progression rate is slow. The MCD diet can simulate the serological and pathological features of NASH in 8 weeks, but no obesity or insulin resistance occurred. The CCl₄ combined with HFD model can induce NASH model in 10 weeks, which can simulate its serological and pathological changes, and the liver has obvious fibrous deposition and oxidative stress damage.