Effect of electroacupuncture on the expression of TRPV4 in the dorsal root ganglion of diabetic neuropathic pain model rats

doi:10. 3969 / j.issn.1005-4847. 2025. 02. 009

Home > Archive>Volume 33, Issue 02, 2025 >85-92. DOI:10. 3969 / j.issn.1005-4847. 2025. 02. 009

Effect of electroacupuncture on the expression of TRPV4 in the dorsal root ganglion of diabetic neuropathic pain model rats
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                        10. 3969 / j.issn.1005-4847. 2025. 02. 009
                    
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                        JIANG Minjian1JIANG Minjian
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China
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CHI Hengyu1CHI Hengyu
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China
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KANG Yurong1KANG Yurong
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China
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JIANG Yongliang1,2,3JIANG Yongliang
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China; 2. Zhejiang Provincial Key Laboratory of Acupuncture Neurology,Hangzhou 310053, China; 3. Institute of Acupuncture and Moxibustion, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
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ZHENG Yinmu1ZHENG Yinmu
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China
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LI Siyi1LI Siyi
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China
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ZHOU Shuting1,2,3ZHOU Shuting
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China; 2. Zhejiang Provincial Key Laboratory of Acupuncture Neurology,Hangzhou 310053, China; 3. Institute of Acupuncture and Moxibustion, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
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LIU Boyu1,2,3LIU Boyu
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China; 2. Zhejiang Provincial Key Laboratory of Acupuncture Neurology,Hangzhou 310053, China; 3. Institute of Acupuncture and Moxibustion, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
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SHAO Xiaomei1,2,3SHAO Xiaomei
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China; 2. Zhejiang Provincial Key Laboratory of Acupuncture Neurology,Hangzhou 310053, China; 3. Institute of Acupuncture and Moxibustion, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
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FANG Jianqiao1,2,3FANG Jianqiao
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China; 2. Zhejiang Provincial Key Laboratory of Acupuncture Neurology,Hangzhou 310053, China; 3. Institute of Acupuncture and Moxibustion, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
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HE Xiaofen1,2,3HE Xiaofen
1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China; 2. Zhejiang Provincial Key Laboratory of Acupuncture Neurology,Hangzhou 310053, China; 3. Institute of Acupuncture and Moxibustion, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
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Affiliation:1. Third Clinical Medical College, Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053, China; 2. Zhejiang Provincial Key Laboratory of Acupuncture Neurology,Hangzhou 310053, China; 3. Institute of Acupuncture and Moxibustion, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China
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Abstract:

Objective To explore the function of electroacupuncture ( EA) on body mass, fasting blood glucose, heat pain threshold, and transient receptor potential vanilloid 4 (TRPV4) in the dorsal root ganglia (DRG) of rats with diabetic neuropathic pain (DNP). Methods A DNP rat model was formed by intraperitoneally injecting the animals with STZ. From days 15 to 21, bilateral Zusanli and Kunlun points of the DNP rat model were treated with electroacupuncture once daily for 30 min. We then measured their body mass, fasting blood glucose, and heat pain threshold. The co-expression of TRPV4 and NeuN in the rat L4 ~ L6 DRG was detected by immunofluorescence. The effects of the TRPV4 agonist GSK1016790A on body mass, fasting blood glucose, and the heat pain threshold of DNP rats treated with electroacupuncture were detected. Results After the 7th day, body mass was significantly decreased (P<0. 01) and fasting glucose was significantly increased (P<0. 01) in the model group compared with the normal group. After the 21st day, compared with the model group, heat pain threshold of the model + electroacupuncture group was significantly higher (P<0. 01); the Results of co-expression of TRPV4 and NeuN immunofluorescence on rat L4 ~ L6 DRG showed that: the expression of positive cells in the model group was significantly higher (P<0. 01) than that in the normal group, the co-expression of TRPV4 and NeuN positive cells in L4 ~ L6 DRG of rats in the model + electroacupuncture group was significantly lower ( P<0. 01) than that in the model group. The TRPV4 agonist GSK1016790A can reverse the downregulation of thermal pain threshold induced by electroacupuncture in DNP rats (P<0. 01). Conclusions Electroacupuncture alleviated the DNP induced by STZ, and its mechanism may involve the inhibition of TRPV4 protein expression in the DRG.

Key words:diabetic neuropathic pain; electroacupuncture; dorsal root ganglia; transient receptor potential vanilloid 4

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Received:August 10,2024
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Online: April 22,2025
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